Gallery

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  • Alcian blue and van giesen staining of mouse growth plate

    Alcian blue/van Giesen staining

    Alcian blue/van Giesen staining is used to distinguish between cartilage (blue) and bone (red). This example is taken from the upper tibia of a 7 week old mouse.

  • Micro CT of mouse femur

    Micro CT of mouse femur

    Micro CT uses x-ray technology to capture micrometre size sections of a bone sample. These sections are then combined digitally to create a 3D image of the bone. This example is from a mouse femur.

  • Calcein double labelling

    Calcein double labelling

    Calcein double labelling uses two fluorescent markers that are introduced into bone as it grows. As the markers are incorporated into newly grown bone it allows researchers to distinguish the regions and rates of bone growth.

  • SEM of trabecular mouse bone

    SEM of trabecular mouse bone

    Scanning electron microscopy (SEM) allows high resolution, high magnification imaging of the internal structure of bones. This example is from the femur of a 14 week old mouse.

  • Psuedo-coloured X-ray of mouse knee

    Pseudo-coloured x-ray of knee

    Pseudo-colour schemes on x-ray microradiographs allow determination between regions of differing bone mineral density. Areas of red, yellow and white are higher in density, while green and blue represents regions of lesser density. This example is from the knee of a mouse.

  • SEM of osteoclast resorption pits

    SEM of osteoclast resorption pits

    Bone marrow osteoclasts cultured on dentine slices resorb mineral at the surface of the dentine leaving characteristic pits. This example is imaged by SEM after 9 days in culture.

  • Psuedo-coloured SEM of trabecular bone

    Pseudo-coloured SEM of trabeculae

    Composite BSE SEM image generated by AutoMontage from a through focus stack. Colours show 3D map contours. This example is shows trabecular bone from the femur of a 14 week old mouse.

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    Actin rings in osteoclasts

    TRITC-labelled phalloidin stain highlights actin filaments in cells. This example shows the actin ring in osteoclast cultures.

  • TRAP staining of osteoclasts

    TRAP staining of osteoclasts

    TRAP staining identifies osteoclasts by detecting tartrate-resistant acid phosphatase. This example is of bone marrow derived osteoclast cells in culture.

  • Triiodide staining of mouse femur

    Triiodide staining of mouse femur

    Triiodide staining allows for visualisation of bone and cartilage in the one image. This example is from the proximal femur of mouse and is imaged by SEM.

  • Safranin O and fast green staining of mouse knee

    Safranin O/fast green stained knee

    Safranin O and fast green staining allows for visualisation of cartilage at the joint as it stains cartilage red and the surrounding tissue green. This example is from the knee of a mouse.

  • X-ray microradiography of mouse knee

    X-ray of mouse knee

    X-ray microradiography is a senstitive and specific scanning technique that allows for calculation of the mineral content in bone. This example is from the knee of a mouse.

  • Quantative SEM in femur

    Quantitative SEM in femur

    An 8 shade pseudocolour scheme allows for quantification of bone mineralisation from BSE SEM images. This example is from a 20 week old mouse femur.

  • SEM of tibeal plateau

    SEM of tibial plateau

    Casts of joint plateaus imaged by BSE SEM allow for microscopic analysis of cartilage damage. This example is from the tibial plateau of a mouse 8 weeks after knee surgery.

  • TRAP staining of histology

    TRAP staining of histology

    TRAP staining identifies osteoclasts by detecting tartrate-resistant acid phosphotase. This example is of a section from near the growth plate of a mouse femur.

  • BSE SEM of joint

    BSE SEM of intervertebral disc

    BSE SEM provides high resolution, high magnification of samples allowing visualisation of a number of anatomical markers. This example shows an intervertebral disc between two vertebrae in the tail of a mouse.

  • Micro-CT of human femur

    Micro-CT of human femur

    Image of the femoral head showing high density mineralised protrusions into the articular cartilage of the hip joint.

  • Feb 2015 Image of the Moment

    Image of the moment – Feb 2015

    February 2015 image of the moment is a microCT of trabecular bone in a mouse femur. Picture from Prof. Duncan Bassett at Imperial College London.

  • March 2015 Image of the Moment

    Image of the moment – March 2015

    March 2015 image of the moment is histology of a mouse tibia. Safranin O/Fast green staining has been used to visualise cartilage. Picture from Dr. Victoria Leitch at Imperial College London.

  • April 2015 Image of the Moment

    Image of the moment – April 2015

    April 2015 image of the moment is a PYY knockout male humerus using quantitative back-scattered electron detection (qBSE). Picture from Dr. Natalie Butterfield and Ms. Joyce Pattara at Imperial College London.

  • Cover of Thyroid

    Thyroid Journal cover

    An image from the OBCD team was used as the cover image for the March 2015 issue of Thyroid.

  • May 2015 Image of the Moment

    Image of the moment – May 2015

    May 2015 image of the moment is a mouse femur undergoing mechanical testing. Picture from Dr. Justyna Miszkiewicz at Imperial College London.

  • June 2015 Image of the Moment

    Image of the moment – June 2015

    June 2015 image of the moment is a 3D reconstruction of trabecular microCT of a mouse femur. Picture from Dr. Justyna Miszkiewicz at Imperial College London.

  • January 2016 Image of the Moment

    Image of the moment – Jan 2016

    January 2016 image of the moment is a montage of psuedo-coloured joints from the WT cohort of the OBCD cartilage pipeline. Picture from Dr. Natalie Butterfield at Imperial College London.

  • May 2016 Image of the Moment

    Image of the moment – May 2016

    May 2016 image of the moment is an image of a cross section from a 3D reconstruction of a microCT scanned WT mouse skull. Picture from Dr John Logan at Imperial College London.